The AMT tracer was synthesized using a high-yield procedure as previously outlined [35].
"You're saying that we would use a high-yield weapon if we have to — or one of the low-yield weapons we already have in our stockpile.
Many bacteria also use a high-yield metabolic pathway in glucose-limited conditions and a low-yield pathway in excess of glucose.
We produced a synthetic gene for IFNT and use a high-yield Pichia pastoris expression system to produce recombinant ovine IFNT (roIFNT) for our studies.
We believe this can be used as a high yield hybrid for production in areas where BPH does not occur.
After insertion of zfAChE cDNA, the enzyme was efficiently expressed in transgenic ZF-L cell lines, which were then used as a high yield source of zfAChE activity for acetylcholinesterase (AChE) inhibition assays.
Using a higher degree polynomial would yield a lower RMS fit error, but could result in undesirable ringing effects in the estimated surface (i.e., Runge's phenomenon) [ 31].
cRNA was synthesized and biotin-labeled using a BioArray high yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY, USA).
cDNA (1 μg) was used as a template for in vitro transcription and biotin labeling reaction using a BioArray High Yield kit (Enzo Life Sciences, Farmingdale, NY).
The gRNA was transcribed using a T7 High Yield RNA Synthesis Kit (NEB) and purified by lithium chloride precipitation following the manufacturer's protocol.
Biotin-labelled cRNA was synthesized from cDNA using a BioArray High Yield RNA Transcript labelling Kit (ENZO, Diagnostics, Farmingdale, NY) and purified by using a GeneChip Cleanup Sample Module (Qiagen Inc., Valencia, CA, USA).
