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The expression of 30 selected miRNAs over 45 tissue-specific and two fetal samples were determined by a EvaGreen based q-PCR approach using a High-Specificity miRNA qRT-PCR Detection Kit (Stratagene, La Jolla, USA) (Table S19).
In our study, using a high-specificity technique, we detected similar aluminum concentrations in the central and peripheral regions of breast tumors.
Using a higher threshold increased the specificity but decreased the NPV (Additional file 2). Figure 3 shows the relationship between the frequency of the high-risk group and the sensitivity, PPV, specificity and NPV.
Due to the assumed very low prevalence in the horse population it is of major importance to use tests with a high specificity to overcome unreliable predictive values.
Due to the very low prevalence of glanders in the horse population it is of major importance to use tests with a high specificity to overcome unreliable predictive values.
Only a few HPV types are needed in an assay to obtain a good sensitivity while maintaining a high specificity using CIN2+ as end point.
A predictive test based on GTL2 alone (using a 1% threshold) has a high specificity (no false positives in this pilot study) but relatively low sensitivity (50% for the OAT and 30% for all samples).
But, using a cut off with a high specificity as suggested in this study, IRT might be a promising tool to detect pigs without clinical symptoms but with lung alterations.
The diagnostic accuracy for selected ABI thresholds (Table 2) showed that the commonly used value of 0.90 has a high specificity but a medium sensitivity in the whole population.
Immunohistochemistry using a mouse monoclonal antibody has a high specificity and sensitivity for detecting IDH1 R132H mutant protein in sections from formalin-fixed, paraffin-embedded tissue.
The Western blot assay indicated that the Nav1.8 antibody used in the IP reaction had a high specificity.
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