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DNA was extracted using a high salt method (Lahiri and Nurnberger 1991).
DNA was extracted from whole blood or lymphoblastoid cell lines using a high salt method.
Genomic DNA was isolated from blood using a high salt phenol/chloroform extraction method as described by Sherman et al. [36].
To remove proteins, fat, polysaccharides, proteoglycans, and insoluble materials, centrifugation after homogenization, and precipitation using a high salt solution and isopropanol was performed as described in the TRIzol® reagent protocol.
After washing, the bound proteins were eluted using a high salt buffer.
Combined samples were cleaned-up using a SCX cartridge (Applied Biosystems) and eluted using a high salt elute of 350 m m KCl and 25% CAN (Applied Biosystems).
Similar(52)
In order to stabilize the p51/p51' homodimer, it was necessary to use a high salt buffer containing 800 mM KCl and 20 mM MgCl2 in addition to the other components.
DNA for genotyping was extracted using a high-salt method [25].
DNA was isolated using a high-salt method, and standard procedures of ethanol precipitation and resuspension in Tris-EDTA (10 mm Tris, 1 mm Na2-EDTA, pH 7.4) and storage at 4°C.
In order to test which of the SAE protein components was more closely associated with STG we explored these linkages using a high-salt stress test, determining Fractional Recovery (FR) values for each protein link.
As templates in the PCR amplifications, genomic DNA was extracted from the fresh tissues of one individual of each given species (Macrobrachium nipponense, Mecistocephalus sp., and Araneus ventricosus) or the whole bodies of several individuals (Macrobiotus areolatus, with body lengths of about 500 µm), using a high-salt method.
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