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The paper reports tests carried out using a high cycle rolling contact test facility based on a thrust ball bearing configuration.
PCR of cDNA, using a high cycle number was carried out to determine if Nhlh2 is expressed in mRNA from either BAT or WAT.
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The half-scale girders (PS-11, PS-12, and PS-13) were intended to be tested under fatigue for 2 million cycles using a high fatigue load level of 10 kips (44.48 kN) to 35 kips (155.69 kN).
Remarkably, it holds a quite stable cycling performance at 45 °C, with capacity retention of 97.4% after 200 cycles using a high rate of 3.0 C, and its low-temperature (−20 °C) specific capacity maintains at high up to 131.4 mAh g−1 at 0.1 C, higher than that of the majority reports.
The resulting cDNAs were PCR-amplified to about 20-30 ng/μl in 18-20 Pcyclesles using a high fidelity DNA polymerase.
With this method, the vector and insert are PCR amplified separately, with only 18 cycles, using a high fidelity DNA polymerase.
PrV transcription was monitored in single cycle conditions using a high MOI that guarantees that more that 90% cells are infected.
The non-normalised cDNA was amplified with 15 cycles of PCR using a high fidelity DNA polymerase.
Primers 5'-CAGTGCTGTCCAGGAGAAGGATTGG-3' and 5'-ATAGGAATAGTTGACACTATGCTGG-3' were used in first round PCR (35 cycles) using a high-fidelity Pfu DNA polymerase (Stratagene).
The mutated scFv gene was re-amplified using a high-fidelity DNA polymerase for 35 cycles.
DNA from each eluted sample was enriched by 18-cycle PCR using a high-fidelity polymerase and a single primer pair corresponding to the Illumina adapters ligated earlier.
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CEO of Professional Science Editing for Scientists @ prosciediting.com