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One of these inhibitors, PF-5168899, was further evaluated using a high content cell-based assay in the presence of CHO cells engineered with GFP-PDK1.
GFP expression for each condition was evaluated and quantified using a high content imaging system and software.
We examined the changes in both cell populations with time using a high content imaging (Fig. 2).
72 hr after seeding, GFP expression and DAPI staining were captured and analyzed using a high content imaging microscope.
In 2003, Aventis published an HTS system for a GJ modulator [ 18] using a high content screening system.
Effects of DECPR on reactive oxygen species (ROS) formation and mitochondrial-initiated events were investigated using a high content screening system.
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It should be noted that using a higher content of silica fume must be accompanied by an adjustment of the water and superplasticizer dosage in the mix in order to ensure that specimens do not suffer excessive self desiccation and cracking.
However, it should be noted that using a higher content of silica fume of more than 7% must be accompanied by an adjustment of the mixing water and superplasticizer dosage of the mix in order to ensure that specimens do not suffer excessive self-desiccation and cracking.
Significantly more gene candidates will undoubtedly be discovered using a higher content, 2nd generation microarray that is currently under development.
Here, we identify the chaperonin CCT/TRiC as a critical regulator of telomerase trafficking using a high-content genome-wide siRNA screen in human cells for factors required for Cajal body localization.
A directed library of 1408 environmental toxicants was screened for Hedgehog/Smoothened antagonist activity using a high-content assay that evaluated the interaction between Smoothened and βarrestin2 green fluorescent protein.
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