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We evaluated compound conversions in shake flasks, using a high cell inoculum at concentrations of aldehydes, which cause 50% of growth inhibition.
It is therefore highly likely that although larger pellets can be generated using a high cell seeding number, optimal ECM synthesis by chondrocytes could only be achieved in smaller pellets where a certain level of oxygen was maintained.
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Fluorescently labeled cells were sorted using a high speed cell sorter.
KSL cells were purified by double FACS sorting using a high speed cell sorter (Dako, Ely, UK).
The key process variables that affect the recovery of products from different sub-cellular locations were investigated using a high throughput cell disruption system and a cyanobacterium as model organism.
The LIBS experiments were performed using a high pressure cell to characterize and test the effectiveness of the donut beam transmitted through the LIBS probe and collect plasma signal in back scatter mode.
The leukocyte suspension and the crude erythrocytes were homogenized by using a high speed cell disrupter.
One of these inhibitors, PF-5168899, was further evaluated using a high content cell-based assay in the presence of CHO cells engineered with GFP-PDK1.
Separation of B and T cells was done using a "High Gradient Magnetic Cell Separation" with Variomacs system (Miltenyi).
In addition, single cells, sorted by using a high-speed cell sorter, were identified by confocal microscopy to be positive for the cell markers CD4 or CD8 and CD20.
Moreover, due to the synergistic effect of using a high temperature fuel cell such as solid oxide fuel cell (SOFC) and a recuperative gas turbine (GT), the integrated system efficiency can be significantly improved.
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