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The blood sample was collected by cardiac puncture using a heparinized syringe under deep anesthesia with 3.0%% isoflurane.
The blood (400 μL) was slowly withdrawn from the cervical vein using a heparinized syringe with a 27-gauge needle.
Arterial and venous blood gases and co-oximetry measurements were collected at baseline, immediately following hemodilution, and at 30 and 60 min following completion of hemodilution using a heparinized syringe.
Fish were bled through puncture of the caudal vein using a heparinized syringe.
Cardiac blood was removed using a heparinized syringe and sera were prepared and stored at −20 degrees.
Cardiac blood was collected from sham-perfused birds at the end of the sham perfusion, using a heparinized syringe.
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Blood was collected from the brachial or cephalic artery or from the vein by using a heparinized 3.0-mL syringe and a 22-gauge or 27-gauge needle and stored for 24 hours at 4°C to allow for plasma separation; the separated plasma was then stored at –20°C until use.
Blood samples were collected from the dorsal vein using a heparinized 38 mm long 18 gauge needle and a 60 ml syringe and transferred to heparinized vacuum tubes.
The plasma blood was withdrawn using a heparinized tube.
Blood was collected from the jugular vein using a heparinized set Omnican® 40 (Braun, Germany).
Upon capture and immediately after RMR measurements (murres) or only immediately after RMR measurements (kittiwakes), birds were blood sampled (1 mL from the alar vein into a heparinized syringe using a 25-gauge needle).
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