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Blood was collected from the jugular vein using a heparinized set Omnican® 40 (Braun, Germany).
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The blood sample was collected by cardiac puncture using a heparinized syringe under deep anesthesia with 3.0%% isoflurane.
The blood (400 μL) was slowly withdrawn from the cervical vein using a heparinized syringe with a 27-gauge needle.
Arterial and venous blood gases and co-oximetry measurements were collected at baseline, immediately following hemodilution, and at 30 and 60 min following completion of hemodilution using a heparinized syringe.
Cardiac blood was removed using a heparinized syringe and sera were prepared and stored at −20 degrees.
Using a heparinized capillary tube, approximately 50 µl of blood was obtained from the saphenous vein of each mouse.
Cardiac blood was collected from sham-perfused birds at the end of the sham perfusion, using a heparinized syringe.
At control sites all individuals captured were measured, ear-tagged and released at capture site, and in addition, whole blood was taken from the retrorbital sinus of the eye using a heparinized capillary tube and Nobuto strips.
Blood samples were collected from the dorsal vein using a heparinized 38 mm long 18 gauge needle and a 60 ml syringe and transferred to heparinized vacuum tubes.
Fish were anesthetized and blood was extracted from the caudal vein using a heparinized syringe.
A sample of blood sampling was collected from each individual by puncturing the caudal blood vessel using a heparinized syringe.
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