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Each blood sample was collected using a heparinized plasma separation tube and centrifuged for 10 min (4°C, 1,581× g) to obtain the plasma.
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Trunk blood was collected using a heparinized syringe, and plasma was prepared by centrifugation at 2,000 rpm for 10 min, then stored at -30°C until use.
The plasma blood was withdrawn using a heparinized tube.
The blood sample was collected by cardiac puncture using a heparinized syringe under deep anesthesia with 3.0%% isoflurane.
The blood (400 μL) was slowly withdrawn from the cervical vein using a heparinized syringe with a 27-gauge needle.
Fish were bled through puncture of the caudal vein using a heparinized syringe.
Cardiac blood was removed using a heparinized syringe and sera were prepared and stored at −20 degrees.
Using a heparinized capillary tube, approximately 50 µl of blood was obtained from the saphenous vein of each mouse.
Cardiac blood was collected from sham-perfused birds at the end of the sham perfusion, using a heparinized syringe.
Blood samples were collected from the dorsal vein using a heparinized 38 mm long 18 gauge needle and a 60 ml syringe and transferred to heparinized vacuum tubes.
Blood was collected from the jugular vein using a heparinized set Omnican® 40 (Braun, Germany).
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