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Male F2 mice were fasted for 4 hours at the beginning of the light cycle (6:00-7:00 AM) and blood was collected via the retro-orbital plexus using a heparinized microcapillary tube at 8, 12, 16, 20, and 24 weeks of age.
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The blood sample was collected by cardiac puncture using a heparinized syringe under deep anesthesia with 3.0%% isoflurane.
The blood (400 μL) was slowly withdrawn from the cervical vein using a heparinized syringe with a 27-gauge needle.
Fish were bled through puncture of the caudal vein using a heparinized syringe.
Cardiac blood was removed using a heparinized syringe and sera were prepared and stored at −20 degrees.
Using a heparinized capillary tube, approximately 50 µl of blood was obtained from the saphenous vein of each mouse.
Cardiac blood was collected from sham-perfused birds at the end of the sham perfusion, using a heparinized syringe.
The plasma blood was withdrawn using a heparinized tube.
Blood samples were collected from the dorsal vein using a heparinized 38 mm long 18 gauge needle and a 60 ml syringe and transferred to heparinized vacuum tubes.
Fish were anesthetized and blood was extracted from the caudal vein using a heparinized syringe.
Blood was collected from the jugular vein using a heparinized set Omnican® 40 (Braun, Germany).
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