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Finally, the resulting paste was casted in a mold and cured using a heating procedure: 80 °C/10 h + 100 °C/3 h + 150 °C/3 h, forming the ER-1% BNNS composite sheets as testing samples.
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The vector was transduced into BL21 Star One Shot Chemically Competent Escherichia coli (Invitrogen) by using a heat shock procedure.
Rectal temperature was maintained at 37.0 ± 0.5°C using a heating pad throughout the surgical procedure.
Breathing was monitored and temperature was maintained using a heating pad throughout the imaging procedures.
The body temperature was kept at 37.0 ± 0.5°C (mean ± SD) using a heating pad (Unique Medical, Tokyo, Japan) throughout the surgical procedure.
During the entire procedure, the animals were kept warm using a heating pad.
Rectal temperature was monitored throughout the surgical procedures and was maintained at 37 °C using a heating blanket controlled by an electronic temperature controller (KN-474; NATUME, Tokyo, Japan).
Throughout all procedures, body temperature was maintained at 37°C using a heating pad.
Normothermia was kept using a heating pad.
Starch removal to obtain dietary fibers is usually accomplished via a three step, sequential enzymatic treatment procedure using a heat stable α-amylase, protease, and amyloglucosidase.
Providing a high-milk diet is a suitable option to help mitigate calf discomfort due to the disbudding procedure, whereas using a heat lamp does not seem to help, at least in a mildly cold winter.
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