Sentence examples for using a heating block from inspiring English sources

The vial was then heated for 15 min at 100°C using a heating block.

Digestions were performed using a heating block (Model 1016, Tecator, Hoganas, Sweden) with an exhaust-collecting manifold.

The [14C] labelled cells were initially resuspended in CH3OH/0.3% NaCl (2 ml, 100∶10, v/v) and mixed with 1 ml of petroleum ether (60 80°C) for 15 min. The upper petroleum ether layer was removed and a further 1 ml of petroleum ether added, followed by further mixing for 15 min. The petroleum ether extracts were combined and evaporated under nitrogen using a heating block.

The mixture was heated at 90 °C for 2 h using a heating block with stirring capacity (Reacti-Therm, Pierce, Rockford, USA).

Briefly, 800 μl of methanol was added to 160 mg (FW) of leaf tissues followed by incubation at 60°C for 45 min using a heating block, then the mixture was centrifuged twice at 12000 rpm for 10 min.

The fresh plant material was dried at 60 °C, weighed, and digested in 70 71 % HNO3 using a heat block.

Four samples (892, 893, 902, and 903) were incubated in buffer ASL for 10 min at 70°C using a heat block in step two to increase DNA yield.

300 ng of the 360 bp amplicon was then denatured by heating to 95°C and slowly reannealed using a heat block to randomly rehybridize wild type and mutant DNA strands.

The reaction was performed at 50 °C for 4 h using a heat block (Thermo Block Rotator SN-06BN; Nissin, Tokyo, Japan) with shaking at 35 rpm, and the supernatant was collected by centrifugation for 10 min at 8000× g at 4 °C to remove cells and debris.

The assay had a detection limit of 0.74 pg of purified target DNA which corresponds 20 copy numbers per reaction within 30 minutes using a simple heating block.

DNA was extracted by inoculating the cultured K. pneumoniae into 50 μl of sterile water and exposing them to 100°C using a microtube heating block (Stuart equipment, UK) for 5 min.