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The design of processing lines using a heat shock to extend the shelf-life of fresh-cut lettuce will need to be modified from existing designs to take full advantage of the effect of the heat treatment.
To induce the over-expression of MTL-1 and MTL-2 at the L2-larval stage, MTL-1 and MTL-2 were expressed in the wild-type N2 animals using a heat shock promoter.
Each recombinant vector was transformed into E. coli BL21 (DE3) using a heat shock protocol.
The vector was transduced into BL21 Star One Shot Chemically Competent Escherichia coli (Invitrogen) by using a heat shock procedure.
The embryonic lethality of pkn can be rescued with a pkn cDNA that is expressed using a heat shock promoter (Betson and Settleman 2007).
This vector was used to transform competent BL21 E. coli cells (Novagen, Madison, WI, USA) using a heat shock (42°C) transformation method.
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This is because the times were derived by clonal analysis using a heat shock-flip, and heat shock causes a long-term increase in ISC division rates in the adult midgut.
Blocking Bmp signalling at the cardiac disc stage by over-expression of the Bmp inhibitor Noggin, using a heat shock-inducible transgenic line, prevented the leftward movement and rotation of the myocardial tissue.
The Saharan silver ant (Cataglyphis bombycina) uses a heat shock protein in a novel way and forages in the open during brief forays in the heat of the day.
For that, we used a heat shock treatment that involved exposing the cell cultures to 50°C for 20 minutes.
To induce adult-specific expression of RPM-1, we used a heat shock promoter (P hsp-16.2 ) that allows temperature dependent induction of gene expression.
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