The samples were scanned using a head coil on a Siemens Magnetom Trio with a 3 T magnet.
Scanning took place in a 3T Bruker MR system using a head coil.
The cadaver head was scanned in a 1.0 Tesla MRI system (Philips Panorama HFO, Philips System, Best, The Netherlands) in prone position using a head coil.
In the latter, one simply uses a head lamp or flashlight to scan the desert floor for beetles walking about at night.
Height and phase images were obtained under ambient conditions with a typical scan speed of 0.5 to 1 line/s, using a scan head with a maximum range of 100 μm × 100 μm.
Multiphoton FLIM-FRET imaging was carried out on a Nikon Eclipse TE2000-U inverted microscope with an Olympus long working distance 20 × 0.95 numerical aperture water immersion lens using a scan head specifically designed for multiphoton excitation.
Photostimulation was carried out using a dual scan head raster scanning confocal microscope and control software developed by Prairie Systems, and incorporated into a BX51 upright microscope (Olympus America).
In the representative extraction experiment shown in Figure 2 bleaching was performed using a confocal scanning head and image acquisition pre- and post-extraction with a CCD camera for optimal sensitivity (see Materials and Methods; [6].
Microfluidic chambers with explants housed inside were placed on an x-y position controlled stage and time-lapse sequences for translocation experiments were recorded using a confocal scanning head (Leica TCS SP5: Leica Microsystems, Bannockburn IL) mounted on an inverted compound microscope.
An image using a bulk scanning head is shown in Fig. 6(a).
