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Both THP-1 and J774 cells were fluorescently tagged using a green fluorescent cell linker mini kit (catalogue number MINI-67; Sigma-Aldrich) [ 21] and then pretreated with PMA as described above.
To determine whether the elo null mutations perturb the localization of WT α-syn, fluorescence microscopy was conducted using a green fluorescent variant of WT α-syn (EGFP-WT-α-syn) after a 6 h induction.
was investigated in 83 biparental crosses involving 39 anthurium cultivars, using a green fluorescent protein-based screening method.
The objective of this work is to develop a novel cleaner dye for leather using a green fluorescent protein (GFP) by genetic engineering approach.
We examined several strategies to develop a Citrus tristeza virus (CTV -based veCTV -basedransient expression of foreign genes in citrus trees using a green fluorescent protein (GFP) as a reporter.
Similar results were obtained using a green fluorescent dye for MA- cells and a red dye for MP cells (dye swap experiment, data not shown).
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The method uses a green fluorescent protein (GFP) covalently fused to target membrane proteins and the resulting fusion proteins are then transiently expressed in insect cells.
They used a green fluorescent protein (GFP) reporter that is specifically degraded by the cellular proteasome.
To better visualize the colonization process we used a Green Fluorescent Protein (GFP) tagged P. brassicae transformant.
We previously used a green fluorescent protein (GFP -tagged strain of Foc TR4 and characterized early events in infection and disease development of Cavendish plantlets [ 19].
We used a green fluorescent protein- (GFP-) expressing Escherichia coli strain as a tracer to find causal association between bacteria present in the gut lumen and the extraintestinal sites.
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