Sentence examples for using a gradient described from inspiring English sources

Samples were analyzed using a gradient described previously [6].

Fractions SP4f6 to SP4f9 (15, 15, 20 and 30%% v/v EtOAc in cyclohexane, respectively) were separately subjected to normal phase HPLC using a gradient mobile phase as described below.

PCR reactions with genomic DNA as a template were carried out as previously described [ 40- 42] using a gradient PCR thermal cycler (Mastercycler Gradient; Eppendorf, Germany).

Both solvents were acidified with 0.1% formic acid and run using the gradient described in the supplementary data.

The stability of the radiolabelled peptide was checked at room temperature first 4 h and then 24 h after the end of radiolabelling by HPLC using the gradient described above.

Fractions collected after the second dimension that showed activity were separated on the HILIC column now coupled to the MS using the gradient described above.

Selected fractions were loaded onto a 1-mL MonoQ 5/5 anion exchange column (Amersham-Pharmacia Biotech, Piscataway, NJ), and RFAP synthase was purified using the gradient described in the detailed protocol.

Ultraviolet photodissociation spectra of the same peptides were acquired on an Orbitrap Elite mass spectrometer (ThermoFisher) after elution using the same gradient described previously on an Eksigent NanoLC Ultra (Eksigent) at a flow rate of 300 nL/min.

One additional step was performed on a narrow bore C18 reversed phase column (150×2 mm, Waters) at a flow rate of 0.2 ml/min using the AcN gradient described in the step 2. The purity assessment and the molecular mass determination of the peptides were carried out by Ultraflex II MALDI-TOF/TOF instrument (Bruker Daltonics, Bremen, Germany).

Correctly folded nanostructures were purified using a previously described gradient ultracentrifugation method.

Unless noted otherwise, elutions performed on these systems used a gradient profile (HPLC method 1, previously described as "Gradient 1") with 0.1% TFA in water (solvent A) and MeOH (solvent B) as solvents.