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These were then aligned using a global alignment algorithm [30].
The Br and At sequences were aligned using a global alignment algorithm (not a blast family algorithm) with no end gap penalties (Needleman and Wunsch 1970).
Subsequently, these sequences were aligned using a global alignment algorithm to remove sequences for which the best blast hit represented only local homology.
If the best hits were the same for both the gene prediction, we re-aligned the two gene models on the reference using a global alignment based on Needleman Wunsch algorithm implemented in the program stretcher from EMBOSS package [ 67].
PE-SW-remap obtained the best mapping results using a global alignment without seeds.
In the following section, we describe analysis of such sequences using a global alignment algorithm to determine the nature of evolutionary modifications that may have contributed to the lack of detectability of these CNSs.
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We wrote a simple perl script to use a global alignment algorithm (Needleman and Wunsch 1970) with cost-free ends (BLOSUM 62) to align the nucleotide sequences of each αCNS without a detectable ortholog in Br with the expected orthologous regions within Br; these regions were found as described previously.
They all use a global alignment algorithm in [ 4] to construct an alignment for the entire length of the sequences.
FINDSITEX on the other hand, as mentioned, employs homology modeling methods to model structures of template proteins, which are then aligned and compared to target proteins using a global structure alignment method.
The top four FASTA hits (Figure 4A) were aligned with the intact or deleted arms using a global sequence alignment method (NEEDLE).
The pair-wise sequence identity of C2 domains was determined using needle, a global alignment tool within EMBOSS [ 29].
More suggestions(15)
using a global matrix
using a global testing
using a spliced alignment
using a global value
using a global navigation
using a global rating
using a global optimization
using a global market
using a global skin
using a structural alignment
using a global grid
using a manual alignment
using a molecular alignment
using a gapped alignment
using a suitable alignment
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