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A microcapilary was tapered with a tip diameter of ca. 10 μm using a glass puller.
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A slit was then made along the dorsal midline using a glass capillary pulled to a sharp point, and the body walls glued flat to the coverslip.
The SE sample was collected using a glass micropipette constructed using glass capillaries (1 mm O.D; WPI Inc, Sarasota, Florida, USA) and a horizontal tip puller (Cat. No. 2001, Scientific and Research Instruments Ltd ,England).
Try using a glass cage.
For pull-down, one brain of an adult P25-P300) mouse (ca. 0.4g) was homogenized in 20 ml using a glass-homogenizer in pull-down buffer completed with protease inhibitors (Roche), 1% Triton X-100, and 2 mM CaCl2.
Don't use a glass.
Only use a glass container.
Immediately after completing the SCI model of rats, 0.5 µl of OECs were stereotaxically microinjected into the spinal cord dorsal column midline, 1.5 mm rostral to the lesion site using a pulled glass micropipette with an inner diameter of 40 µm.
The surrounding fat and tissue were removed using a pulled glass capillary pipette.
Approximately 2 5 µl of DNA solution was injected into the lateral ventricle at the indicated ages using a pulled glass micropipette.
MO oligonucleotides were injected into the hindbrain lumen of 22 25 somite-old embryos (14 15 Hamburger Hamilton stage) by using a pulled glass capillary.
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