Sentence examples for using a glass potter from inspiring English sources
All of the tissues were mechanically lysed at 4°C in CHAPS buffer [CHAPS 4%, 5 mM Tris, pH 8.8, 0.05% protease inhibitor cocktail (Sigma)] using a glass potter, and the samples were centrifuged at 2000 g for 15 minutes at 4°C in order to eliminate aggregates and debris and then stored at −20°C.
Hearts were homogenized in 10 volumes of PBS containing Complete™ using a glass potter Elvehjem homogenizer.
PVAT was homogenized in Krebs Ringer buffer using a glass potter Elvehjem homogenizer and protein concentrations were measured with the Thermo Scientific Pierce BCA™ Protein Assay Kit.
Frozen hearts (40 50 mg) were homogenized with 8 volumes of a chilled mixture containing 100 mM HClO4 and 85 mM H3PO4 using a glass potter Elvehjem homogenizer.
Tissues were homogenized in a solution containing 0.25 M sucrose, 1 mM EDTA, 1 mM dithiothreitol, and Complete™ (pH 7.0) using a glass potter Elvehjem homogenizer, and homogenates were centrifuged at 20,000 g for 30 min at 4 °C.
Hearts were homogenized in 10 volumes of a 50 mM triethanolamine/HCl buffer (pH 7.4), supplemented with 2 mM dithiothreitol, 2 mM EDTA and Complete™, using a glass potter Elvehjem homogenizer.
The resulting microsomal protein fraction was homogenized in 2 mL TEG buffer (50 mM Tris HCl, 1 mM EDTA, 30%% w/v glycerol) using a glass homogenizer (Potter-Elvehjem, Fisher Scientific, Schwerte, Germany).
For further treatment, tissues were rethawed in a 30-fold volume of ice-cold 1% (v/v) trifluoracetic acid, and homogenized at 4°C using a glass-teflon potter (Satorius-S, Göttingen, Germany).
Hippocampi were homogenized using a hand glass potter and total RNA extracted using the SVtotal RNA Isolation System kit following the manufacturer's instructions (Promega, Madison, WI, USA).
Testis samples were homogenized using an hand glass potter, and total testis RNA extracted using the SVtotal RNA Izolation System kit (Promega, Madison, WI, USA).
