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Ultrathin sections of the pelleted samples were cut on an RMC MT-X ultra-microtome using a glass knife.
Blocks were trimmed using a glass knife and 70 nm sections cut using a diamond knife.
Semi-ultra thin sections (2 4 µm) were prepared using a glass knife and stained with the methylene blue for examination under the microscope LEICA (Model DM LB2).
Embedded samples were first trimmed with a razor blade and then 2 µm thick sections were cut using a glass knife mounted on an ultrotome.
Beating outgrowths from D3 murine ESC-derived embryoid bodies (day 8) and human iPSC- derived embryoid bodies (day 30) were micro-surgically dissected using a glass knife, followed by incubation in collagenase B (1 mg/ml)(Roche) at 37°C for 30 minutes with occasional dispersion by pipetting up and down.
Semi-thin sections were prepared using a glass knife and mounted on Superfrost Plus glass slides (Thermo Shandon).
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The material was sectioned with a glass knife using a Leica UC6 ultramicrotome (Leica).
For the cotyledon cross-sections, the embedded pieces were sectioned using an automated ultra-microtome and a glass knife.
This knife maker provides a glass knife for semi-thin and ultra-thin sectioning with ultramicrotome.
Thereafter 0.5 3.0- μm-thick sections were cut with a glass knife.
The blocks were sectioned with 1 3 μm thickness using a Reichert ultramicrotome glass knife and stained with toluidine blue solution in 50% ethanol to determine the area of interest.
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