Sentence examples for using a glass homogeniser from inspiring English sources

Eyes were homogenised in sterile PBS with protease inhibitors (Sigma Aldrich, Gillingham UK) using a glass homogeniser.

Working on ice, meninges and cerebellum + brain stem were carefully removed, the remaining brain tissue minced into small pieces and homogenised in culture medium (DMEM/F12 containing l-glutamine 2 mM, FCS 10%, PenStrep 1.0% (all from Invitrogen, Switzerland)) using a glass homogeniser with Teflon pestle (Wheaton, USA).

Muscle aliquots were homogenised in buffer, using a glass on glass homogeniser, and centrifuged at 1,500 × g for 5 min at 4°C to remove insoluble connective tissue.

Working on ice, brain materials (hippocampi and cortices) were homogenised separately in homogenisation buffer [Tris-HCl 10 mM, pH 7.4, EDTA 1.0 mM, sucrose 250 mM and protease inhibitors (Complete®, Roche, Switzerland)] with 30 strokes using a glass Dounce homogeniser with Teflon pestle (Wheaton, USA).

Non-necrotic (1 2 cm) tumour masses were homogenised in PBS, 0.5% Nonidet P-40, 10  μg ml−1 soybean trypsin inhibitor, 5  μg ml−1 leupeptine, 8  μg ml−1 aprotonin and 0.5 m M PMSF and homogenised using a glass-teflon homogeniser.

Cells were disrupted using a glass hand-held homogeniser (40 passes), and the lysate was clarified by centrifuging for 10 min at 800 g at 4°C.

Try using a glass cage.

Pellets were resuspended in 20 mM Tris HCl (pH 7.5 /150 mM KCl/1% sarkosyl and lightly homogenised using a ground glass homogeniser.

Lysates of D. immitis and O. ochengi were prepared using a small glass homogeniser containing 100 μl of ice-cold TNES and the supernatant processed as described above. 1 mg GA (InvivoGen) was derivatised using 1,6-hexanediamine to yield 17-hexamethylenediamine-17 17-hexamethylenediamine-17 17-hexamethylenediamine-17

Between washes thylakoids were resuspended and homogenised in a glass homogeniser and centrifuged for 5 min at 7500×g.

The plasma membranes in suspension were disrupted with a teflon pestle in a glass homogeniser and further incubated for 10 min on ice.