Rather, detailed analysis of the seminiferous tubules using a germ cell-specific marker revealed that depletion of spermatogonia was the first step in the degenerative process and led to disruption of spermatogenesis, which resulted in eventual tubule degeneration.
To clarify the key stage for oocyte over-loss after Atg7 deletion, we examined the germ cell numbers by immunohistochemistry using a germ cell molecular marker mouse vasa homolog (MVH) in prenatal and postnatal Atg7 F/F ;Tnap-cre ovaries.
The loss of germ cells was confirmed using a germ-specific antibody (anti-germ cell nuclear antigen (GCNA)) [ 20], which revealed a dramatic reduction in germ cells (GCNA-positive cells) in Cbx3 hypo / hypo testes compared with wild-type mice.
First, we used a germ-free approach to investigate microbiota involvement.
The nuclei were collected, and their purity determined by brightfield microscopic inspection after staining in Trypan Blue solution or by fluorescent microscopic inspection using a wheat germ agglutinin (conjugated to Alexa Fluor® 555) plasma membrane stain in combination with DAPI (4′,6-diamidino-2-phenylindole) nuclear staining.
Federal officials used a benign germ related to anthrax to demonstrate the possible effects.
This was confirmed by immunofluorescence staining of ovaries isolated at PND3, using an antibody to the cytoplasmic germ-cell marker Vasa (Ddx4).
Steam, boil or use a disinfectant to remove germs from pacifiers, baby toys and bottles and nipples.
FOR example, if terrorists were to use a crop duster to spread germs, they would face a number of problems.
If you are using a pencil, make sure to sharpen it every time before use to get germs off.
