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Cytological samples were obtained from CSF using a gentle sedimentation technique.
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All BLX instruments were used to the full WLs using a gentle in-and-out motion.
Solvent was removed by evaporation using a gentle stream of N2 gas.
The samples were then transferred to acetonitrile using a gentle flow of nitrogen.
OPCs were purified using a gentle modified washdown procedure as previously described (Feigenson et al., 2009).
Wash using a gentle hand wash cycle.
Exfoliate three times a week using a gentle exfoliation wash.
Using a gentle soap without harsh chemicals is advised.
Instead it recommends using a gentle cleansing conditioner.
Clean the area using a gentle detergent solution.
Applying gentle pressure with the reins or using a gentle whip tap can be used as negative reinforcement.
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