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Next, the supernatant was transferred to another tube and evaporated using a gentle nitrogen (N2) stream (45 ± 5°C).
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The organic layer was transferred into a clean, silanised, glass vial and evaporated at 40°C using a gentle stream of nitrogen gas.
These solvents were mixed and then evaporated using a gentle stream of nitrogen, followed by drying under vacuum for at least 12 hours in order to remove completely the residual organic solvent.
This solvent was then evaporated using a gentle stream of nitrogen, followed by drying under vacuum for at least 12 hours in order to remove completely the residual organic solvent.
The samples were then transferred to acetonitrile using a gentle flow of nitrogen.
The supernatant was transferred to an Eppendorf vial, and acetonitrile was removed by using a gentle flow of nitrogen.
Iso-octane (2 mL) was added and the reaction mixture evaporated to approximately 1.0 mL using a gentle stream of nitrogen gas.
The hexane layer containing PCBs was collected and concentrated to 2 mL using a Heidolph Synthesis1 evaporator (Heidolph, Schwabach, Germany) and then to 100 µL using a gentle stream of nitrogen.
The acid herbicide extracts were similarly evaporated and transferred to a test tube and evaporated to dryness using a gentle stream of nitrogen gas; the extract residue was then dissolved in acetone (4 mL).
The microwave conditions were as follows: power, 100%; extraction temperature, 100°C; extraction time, 30 min. After the extraction, samples were filtered and evaporated close to 0.1 mL, using a gentle stream of nitrogen.
The mica surface was thoroughly rinsed with deionized water (Milli-Q Watersystem, Millipore Corp. Bedford, MA) to remove excess salt, and then dried using a gentle stream of nitrogen gas.
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