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The integration of this plasmid into the MiMIC elements using a genomic source of PhiC31 is very efficient.
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The genomic DNA from B. licheniformis CGMCC 2876 was extracted using a genomic DNA extraction kit (Omega, China).
Three sources of SSR markers were used: a) EST-SSRs developed ' in silico' specifically for this study, b) genomic SSRs developed by Nunome et al. [ 27], and c) genomic SSRs developed using a genomic library [ 36].
Each library was constructed using a separate heterogeneous source of genomic DNA.
Fungal genomic DNA was extracted using a Plant Genomic DNA Extraction Kit (Tiangen Biotech Co., Ltd., China).
Genomic DNA was then extracted from these 1-week-old seedlings using a Plant Genomic DNA Purification Kit (GeneMark, Taiwan).
Genomic DNA of Acetobacter sp. CCTCC M209061 was extracted and purified using a bacterial genomic DNA Kit.
This study is the first application that uses an entire genomic marker set as source of input information for genome-enabled prediction of milk traits in dairy cattle.
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Methods using multiple genomic data sources have also been proposed [ 9, 10].
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