Therefore, using an mcrBC+ E. coli strain as a host to construct a genomic shotgun library, heavily methylated repetitive DNA is efficiently counter-selected, while hypomethylated low copy (i.e. genic) sequences are substantially over-represented.
The initial objective of the M. perniciosa Genome Project was to obtain a genome survey sequence using a whole shotgun strategy to provide genomic information for the WBD research community.
R. flavefaciens FD-1 genomic DNA was sequenced at the 'University of Illinois in-house genomic facility' using a shotgun sequencing approach.
Here, we used high-throughput genomic shotgun data and qPCR to characterize the germline and somatic genomes of Mesocyclops edax, a freshwater cyclopoid copepod species with a diploid germline genome of ~15 Gb and a diploid somatic genome of ~3 Gb [ 22].
Our study used genomic shotgun sequence data from the cyclopoid copepod Mesocyclops edax to identify the repetitive sequences present in the species' massive germline genome, but eliminated from the somatic genome by chromatin diminution.
Our study used newly generated genomic shotgun sequence data from the basal salamander lineage C. alleganiensis, in combination with previously published data from plethodontid salamanders, to identify genomic features likely to be shared across all crown salamanders.
We used low-coverage genomic shotgun sequence data from four salamander species to examine patterns of insertion, deletion, and substitution in neutrally evolving non-long terminal repeat (LTR) retrotransposon sequences.
Here, we describe and evaluate the reconstruction of this small QTL-rich, sub-genomic region (HICF contig 23) of T. cacao using a pooled BAC shotgun sequencing and assembly approach.
MDA-amplified genomic DNA from the five individual AOA cells was sequenced using a combination of shotgun (SG) and paired-end (PE) DNA pyrosequencing approaches on the Roche/454 FLX Titanium platform.
Next, using a shotgun approach, we divide the RNA into overlapping fragments and probe these fragments.
