An approximately 300-bp length cDNA library was prepared using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Cluster Generation kit (ver. 2) on an Illumina cluster station (Illumina), according to the manufacturer's instructions.
A STRUCTURE analysis was completed using a genomic DNA sample from Nancy, and genotypes from 18 of the 36 short tandem repeat (STR) loci were used to resolve genomic distinctions among wildcat subspecies effectively[ 3].
An ≈300-bp length DNA library was prepared from a mixture of 2 µg of total DNA and ds-cDNA by using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Single Read Cluster Generation kit version 4 on an Illumina cluster station (Illumina) according to the manufacturer's instructions.
DNA was extracted from EDTA treated blood samples using a Genomic DNA mini kit (Geneaid, Taiwan) according to the manufacturer's protocol.
DNA was extracted from pharyngeal swab samples using a Genomic DNA Prep Plus kit (A & A Biotechnology, Gdynia, Poland) according to the manufacturer's instructions, and the extracted DNA was quantified by spectrophotometry (BioPhotometer, Eppendorf, Poland).
Some problem samples were extracted using a Genomic DNA Mini Kit I Multi (Animal/Plant) (Koma Biotech Inc).
Methods and Results: Microsatellite loci were enriched in a genomic sample and isolated using pyrosequencing.
Paired-end libraries with approximate average insert lengths of 200 base pairs were synthesized using the Genomic Sample Prep kit (Illumina, San Diego, CA) according to manufacturer's instructions.
A recent study using extensive genomic sampling (McCluskey and Postlethwait, 2015) to examine relationships within a ' D. rerio species group' identified the poorly known D. aesculapii (Kullander and Fang, 2009) as a candidate sister species to zebrafish.
