Sentence examples for using a genomic reference from inspiring English sources
Results: Using a genomic reference and RNA-seq high-throughput sequencing datasets, supersplat empirically identifies potential splice junctions at a rate of ∼11.4 million reads per hour.
To overcome a bias in alignment of short reads, where the reference allele reads are preferentially aligned over reads with the variant allele, we used a genomic reference containing IUPAC codes for SNPs in dbSNP.
Though the microarray data produced in this study are two-colour, the use of a genomic reference allowed the use of the RankProd package analagously to one-colour data, the values compared being log2 expression ratios of sample_cDNA/gDNA.
The objectives of this study were to investigate (1) the impact of the size of a genomic reference population using German reference bulls on the estimation of SNP effects and on direct genomic values (DGV) and (2) the impact of including an RPG effect on the accuracy of genomic prediction using EuroGenomics reference bulls.
Positive control reactions were performed using a reference genomic DNA purified from axenic cultures of L. (L).
For each run a standard curve was generated using a reference genomic DNA isolated from IVF-derived ESC diluted to 0.06 40 ng per well (5-fold dilution).
There are several others, including: de novo transcriptome assembly [ 3] and de novo alternative splicing calling [ 4] from transcriptomic NGS data (RNA-seq); metagenome assembly [ 5] from metagenomic NGS data; and genomic variant detection [ 6] from genomic NGS data using a reference genome.
Genetic evaluation of dairy cattle enhanced with genomic information is highly effective in increasing reliability, as well as using large genomic reference populations.
Similar results were also reported in mouse and dog aCGH studies that used a reference genomic DNA of an animal of the same breed/line of others that have been analysed for CNV discovery [ 39, 45].
Type 2 microarray experiments using a common reference such as genomic DNA allows for inter-conditional comparisons.
Gene coverage and expression analysis was further investigated in the non-model context by using increasingly divergent genomic reference species to group assembled contigs by unique genes.
