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Target sequences of ATAF2 were identified using a genomic pull-down assay described previously [ 18].
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To further understand the role of ATAF2 in virus biology we utilized a genomic pull-down assay to identify potential ATAF2 target sequences from the Arabidopsis genome.
To detect potential ATAF2 transcriptional targets, a genomic pull-down assay was utilized to identify ATAF2 promoter binding sequences.
Rac1 activity was measured using a Active Rac1 Pull-Down and Detection Kit (Pierce).
The library was enriched for genomic fragments containing microsatellite loci using a standard pull-down method with streptavidin-coated magnetic beads and 3′-biotinylated oligonucleotides carrying repeat motifs (TA 17, (GA 17, (TC 17, (TTC 7, (TACA 7, and (TCAA)7.
Rap1 activity was determined using a Rap1 pull-down assay kit to isolate activated (GTP-loaded) Rap1 molecules (GST-RalGDS-RBD pull-down assay, Thermo Scientific).
Their direct interaction was also examined using a GST pull-down assay.
Using a PI pull-down assay, we observed that MK2206 markedly impaired phosphoinositide binding.
Using a GST pull-down approach, we showed that recombinant fragments of M3R could bind to the Gβ5-RGS7 complex.
p28 binding to fragments of p53 was analysed using a GST pull-down assay (Yamada et al, 2009).
p28-binding regions on p53 were mapped using a GST pull-down assay (Yamada et al, 2009).
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