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Through an inverse metabolic engineering approach using a genomic library of S. cerevisiae, XYL2 was identified as an overexpression target for improving xylose metabolism.
Although alcohol tolerance in yeast is a complex trait affected by simultaneous interactions of many genes, our results using a genomic library reveal potential target genes for better understanding and possible engineering of metabolic pathways underlying alcohol tolerance phenotypes.
Using a genomic library enrichment strategy, we identified approximately 270 genes that were enriched or depleted in n-butanol challenge.
Using a genomic library enrichment strategy, we identified genes involved in n-butanol tolerance in E. coli.
Methods and Results: Fifteen polymorphic microsatellite loci were developed and characterized in S. platyclados using a genomic library enriched for dinucleotide (CT) repeats.
Using a genomic library of Paenibacillus thiaminolyticus, constructed in E.coli DH5α cells, nucleotide sequence of a new α- l-fucosidase isoenzyme was determined and submitted to the EMBL database (HE654122).
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DNA was purified by a classical phenol chloroform protocol and 10 µg used to construct a genomic library using the CopyControl™ Fosmid Library Production Kit (Epicentre) as described previously (Martin-Cuadrado et al. 2008).
A paired-end cDNA library was synthesized using a Genomic Sample Preparation Kit (Illumina, CA, USA) according to the manufacturer's instructions.
Here, we describe our newly developed method for isolating functional MHC class II genes without the use of a genomic library or physical mapping.
The basic I-TRAP approach uses a genomic DNA library in a plasmid vector containing a promoterless 'operon' with two reporter genes.
A study by Reyes et al. [ 37] used a genomic enrichment library to find genes from E. coli that enhanced n-butanol tolerance.
More suggestions(15)
using a genomic reference
using a genomic pull-down
using a synthetic library
using a siRNA library
using a genomic relationship
using a genomic array
using a genomic source
using a new library
using a JavaScript library
using a lipid library
using a phage library
using a genomic approach
using a large library
using a genomic search
using a huge library
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