DNA samples were extracted from cells using a genomic DNA purification kit (Gentra, Minneapolis, MN, US) and separated on a 0.65% agarose gel.
The genomic DNA from B. licheniformis CGMCC 2876 was extracted using a genomic DNA extraction kit (Omega, China).
Purification of DNA was achieved using a Genomic DNA purification kit (Fermentas, GmbH, St. Leon-Rot, Germany) according to the manufacturer's instruction.
The fungal strain, B47-9, was identified by analyzing the genome DNA sequence of internal transcribed spacer (ITS) gene for 18S rRNA as follows: Genomic DNA was extracted using a Genomic DNA Purification Kit (Promega, Fitchburg, WI, USA) according to manufacturer's instructions.
Genomic DNA from CaCo2 (human colon cancer cell line), HepG2 (human hepatocellular carcinoma cell line), and HCT116 cells (human colon cancer cell line, China Infrastructure of Cell Line Resources Chinese Academy of Medical Sciencess, Beijing, China) were extracted using a genomic DNA extraction kit according to the manufacturer (TIANGEN, Beijing, China).
Absolute quantification was determined using a genomic DNA standard curve.
DNA was also extracted using a genomic DNA extraction kit (G-500, Qiagen).
The genomic DNA was isolated using a Genomic DNA Purification Kit (Gentra Systems, Minneapolis, Minnesota).
DNA from these outbred offspring was extracted using a Genomic DNA Purification Kit (Promega, Madison, WI, USA).
To obtain the cDNA fragment expressed in the HmN-2 cells, PCR was performed using a genomic DNA prepared from the HmN-2 cells.
The bacterial chromosomal DNAs were extracted from cells that had been cultured overnight by using a genomic DNA preparation kit (Tiangen, China) with lysostaphin at 10 mg/ml and RNaseA at 25 mg/ml for the lysis step.
