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The genomic DNA was sequenced using a Genome Sequencer FLX/Titanium instrument (454 Life Sciences).
Total genomic DNA was sequenced using a Genome Sequencer FLX System (454 Life Sciences, Branford, CT, USA) at the McGill University and Genome Quebec Innovation Centre (Montreal, QC, Canada).
The genomic libraries were sequenced using a Genome Sequencer FLX + System (Roche) and Illumina HiScanSQ Genome Analyzer (Illumina Inc). in the DNA Sequencing and Oligonucleotide Synthesis Laboratory (oligo.pl) at the Institute of Biochemistry and Biophysics, Polish Academy of Sciences.
Another pooled sample (1032 RCA products) was sequenced using a Genome Sequencer 20 System (454 Life Sciences/Roche).
Following elution from the preparative gel, this size-selected cDNA was sequenced using a Genome Sequencer™ (GS FLX Titanium Instrumentt (Roche Diagnostics) following a standard protocol [ 59].
Sequencing was performed using a Genome Sequencer FLX using a GS Em PCR Kit II (Roche Applied Science).
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The library was validated, quantified and subjected to deep sequencing using a Genome Analyzer IIx Next generation sequencer on a 66 cycle single-end sequencing run, following the supplier's instructions (Geneworks, Adelaide).
Genome sequencing was performed using a 454 Genome Sequencer (Roche) using either GS-20 or Titanium chemistries.
Whole-genome shotgun sequencing was performed using a 454 Genome Sequencer (GS) FLX-Titanium (Roche Diagnostics, US).
The remainder of the sample was used for pyro-sequencing using a Roche/454 Genome Sequencer FLX system.
Approximately 5 μg of each cDNA library was sequenced using a 454 Genome Sequencer with FLX Titanium chemistry (454 Life Sciences).
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