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After washing and boiling, the hybridized DNA was amplified by PCR for a minimal number of rounds and analyzed using a Genome Analyzer II (GAII) sequencing system (Illumina).
The prepared flowcell was then subjected to sequencing by synthesis (version 1.0) using a Genome Analyzer II, according to the manufacturer's instructions.
In order to evaluate these methods, we prepared sequencing libraries from three HapMap samples using both methods for the same target region and sequenced the libraries using a Genome Analyzer IIx instrument (Illumina).
Sequencing was carried out using a Genome Analyzer II (Illumina).
The cDNAs were sequenced using a Genome Analyzer IIx System (Biomarker technologies CO., LTD, Beijing, China).
The florescent light signals were detected using a genome analyzer, which was used for base calling [ 25].
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Cluster generation and 36 bp end sequencing was performed, by the UCL Genomics Core Facility, using an genome analyzer (GAIIX; Illumina) in accordance with the manufacturer's recommendation.
Sequencing was performed using a Illumina Genome Analyzer at BGI ShenZhen (China).
Paired-end sequencing was performed using a GAIIx Genome Analyzer (Illumina).
Following the first run, clusters were resynthesized with the Paired-End Cluster Generation Kit v4, and paired-end reads were obtained using a second Genome Analyzer run.
Finally, the libraries were used for clustering and sequencing using an Illumina Genome Analyzer II (Illumina, San Diego, CA).
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