Response 2: To validate that the effects of cavin-3 siRNA treatment were due to loss of cavin-3 as opposed to off-target effects, we chose to test whether loss of cavin-3 had similar effects in different model systems in which loss of cavin-3 resulted from different means: the MEF model uses a genetic knockout of the cavin-3 locus and the H1299 model is an epigenetic hypomorph of cavin-3.
Rubin and coworkers performed a series of studies using a genetic technique known as Transposon Site Hybridization (TraSH), where a random insertion of this mobile genetic element is made to knockout a gene (17 19).
We used a conditional genetic knockout of SMC2 in DT40 SMcellsOFF cells (Hudson et al., 2003) to compare the composition of mitotic chromosomes formed in the presence or absence of condensin, which is required for structural integrity of mitotic chromosomes.
The goal of this study was to test the feasibility of using fetal liver-derived cells (FLDC) to perform gene targeting, and create a genetic knockout pig.
To further confirm the suppressive effects of Eph receptor signalling on spinogenesis in the proximal domain of Purkinje cell dendrites, we used a genetic approach and analysed EphB knockout mice [7].
To generate mice characterized by a genetic knockout of the rac1 gene in liver, we made use of the Rac1flox/flox/Mx1-Cre strain described before.
To obtain chromosomes depleted of Ska complex, we isolated a genetic knockout of the Ska3/Rama1 gene.
For example, OptGene uses a genetic algorithm to search the vast solution space to obtain optimal knockout sets.
We did, however, perform functional experiments using RNAi knockdown of both Nrp1 and Nrp2 in NIH3T3 cells, and using genetic knockout (Nrp2 complete knockout; Nrp1 conditional knockout) in GNPs in the developing cerebellum.
In order to examine the role of GTS1 in plant growth development, we employed a reverse genetic approach using a SALK_TDNA knockout insertion (Salk_010647) of GTS1 gene to investigate the effect of loss of GTS1 function in gts1 mutant.
