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Loci containing the critical genes were mapped by Linkage Group Selection, using a genetic cross between the high-level chloroquine-resistant mutant and a genetically distinct sensitive strain.
Using a genetic cross with Azucena, we mapped Bsdr1 onto a 30.8 cM region on chromosome 12.
Knockout mutants that were deposited at the FGSC as heterokaryons (NCU09796, NCU00700, and NCU08429) were purified to homokaryons using a genetic cross to wild-type strain FGSC2489, as described (Colot et al. 2006).
Using a genetic cross between these morphs, we discovered significant correlations among several cave-related traits, including reduced eyes, increased superficial neuromast receptors located within the eye orbit (EO SN) and a vibration-attraction behavior (VAB) that facilitates foraging in darkness.
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Comparisons with other mouse phenotype datasets, often aimed at identifying contrasting biological variables in a pair of strains used in a genetic cross, remain difficult due to differences in genetic backgrounds, technical assays and maintenance conditions that inevitably induce phenotype variations, even in self contained experiments [28].
Here we identified the leuA gene encoding α-isopropylmalate synthase in the zygomycete fungus Phycomyces blakesleeanus using a genetic mapping approach with crosses between wild type and leucine auxotrophic strains.
To investigate this possibility using a genetic approach, APP tg mice were crossed with a line of mice that is heterozygous deficient for endogenous murine Cdk5 gene (CDK5+/−).
Using a genetic approach, the biological relevance of the ATXN1/Tip60 interaction was assessed by crossing ATXN1[82Q] mice with Tip60+/−animals.
In a previous such analysis using the progeny of a genetic cross between P. c. chabaudi lines AS-pyr1 and CB, a location on P. c. chabaudi chromosome 8 containing the gene for merozoite surface protein-1, a known candidate antigen for Strain Specific Protective Immunity, was strongly selected.
This strategy may be particularly suited to neurodegenerative disease models, where performance in a simple motor-coordination task would provide the baseline data and used to identify outliers in a genetic cross with a population of random mutants (16).
To systematically delineate the signal pathways downstream from ROS we used a genetic approach and crossed mutants related to plant hormones (ABA, JA, SA, ethylene and auxin) and defense signaling pathways into cat2 background (see Additional file 1: Table S1 for an overview of the genotypes used).
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