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The PCR products were analyzed using a genetic analyzer (ABI310; Applied Biosystems).
Purification of sequencing reactions and capillary electrophoresis were performed in the DNA sequencing core facility at the University Hospital of North Norway (Universitetssykehuset Nord-Norge, Tromsø, Norway) using a Genetic Analyzer 3130xl (Applied Biosystems®).
Electrophoresis and size determination of the amplified DNA fragments were performed using a Genetic Analyzer 3700 (ABI) and GENESCAN (ABI), respectively.
PCR products were sequenced in both directions either at Agencourt Biosciences, Inc. (Beverly, MA), or at the ONPRC using a Genetic Analyzer 3130 (Applied Biosystems , Inc.
DNA sequences of the PCR products were directly determined using a Genetic Analyzer (ABI PRISM TM 310, PE Applied Biosystems) after termination-dideoxy-cycle sequencing (Sequencing Reaction Kit-FS, PE Applied Biosystems) with the forward primer (NOT-LR-F).
To fill in the gaps between contigs, a total of 95 primers have been designed and used for sequencing by Sanger technology using a Genetic analyzer 3130 (Life Technologies, Carlsbad, USA).
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Samples were analyzed using a 3100 Genetic analyzer (Applied Biosystems).
The mixture was heated to 90°C for 2 minutes, chilled on ice for 5 minutes and then used for electrophoresis, using an ABI310 genetic analyzer (Applied Biosystems).
DNA sequence of the resulting amplicon was confirmed by sequencing using a 3130xL Genetic Analyzer (Applied Biosystems, CA, USA).
PCR products were sequenced using a 3130xl Genetic Analyzer (Applied Biosystems).
Sanger sequencing was performed using Big Dye reagents (ABI) and subjected to chromatography using a 3730 Genetic Analyzer (ABI).
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using a diagnostic analyzer
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using a static analyzer
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