Sentence examples for using a gene probe from inspiring English sources

Although ERα gene amplification in breast cancers is controversial [ 41], we performed FISH analysis on tissue microarrays (TMAs) with known AR-positive breast cancers using a gene probe for AR and a centromeric chromosome X probe to query for AR gene amplification.

Briefly, RNA samples (200 ng total RNA) were first reverse-transcribed using a gene-specific probe mix.

The hrk1Δ mutant strain was confirmed by both diagnostic PCR and by Southern blot analysis using a gene-specific probe prepared by primers listed in supplemental Table S1 (Fig. S2).

We confirmed the mutation by Southern blot hybridization using a gene-specific probe and tested the strain for cheating.

To investigate RAP2.1 expression patterns in response to different abiotic stresses, northern blot analysis was conducted using a gene-specific probe for RAP2.1.

38 In another recent study, a panel of lung cancer cell lines was probed using a gene signature of EMT, and the cell lines that appeared more mesenchymal also showed greater resistance to epidermal growth factor receptor and PI3K/Akt pathway inhibitors.

The Rotor-Gene SYBR Green PCR kit (Qiagen) was used for Real-Time PCR expression profiling of icaA[ 25] and gyrB (reference gene; [ 26]) using a Rotor Gene Probe PCR Kit (Qiagen).

Liquid based cytology, which is presently assessed in some UK laboratories, allows adjunctive HPV testing using a commercial gene probe HPV DNA Hybrid Capture II (Digene/Abbott, Maidenhead) without the need for an additional specimen.

Southern analysis of Euglena genomic DNA using a tRNAArg gene probe identified multiple hybridizing bands (12 in BamHI/ EcoRI, ranging in size from 2.1 kbp to 16 kbp; Fig. 4A), suggesting that the tRNAArg gene is also multi-copy in the Euglena genome.

Finally, to elucidate the molecular mechanisms underlying AQP5 expression in CML cells, we investigated the presence of genomic amplification through fluorescence in situ (FISH) analysis using an AQP5 gene probe.

Ballerstedt et al. ([2007]), using specific gene probe of P. putida KT2440 in a microarray identified 67.8-10067.8-100%al genes in six P. putida strains.