Sentence examples for using a gene chip from inspiring English sources
Gene identification was achieved using a gene chip approach and was corroborated by real-time PCR.
By using a gene chip to compare the salivary RNA of people in each disease group to that of 10 healthy people, the researchers found candidate RNA markers linked to Sjogren's syndrome (26 RNAs), type II diabetes (126 RNAs), and breast cancer (103 RNAs).
The WPMY-Vec and WPMY-AR cells were then profiled for overall gene expression patterns and for changes in these patterns associated with DHT treatment using a gene Chip microarray approach.
The direct labeling procedure was used with 1 μg of total RNA sample; double-stranded cDNA was synthesized with a T7 promoter-containing oligo (dT) primer using a Gene chip one-cycle cDNA synthesis kit (Affymetrix), followed by in vitro transcription using a Gene chip IVT labeling kit (Affymetrix).
The Roche test, the AmpliChip CYP450, uses a gene chip made by Affymetrix and looks for mutations in two genes that are involved in metabolizing up to 25percentt of existing drugs.
In this case, scientists used a gene chip developed by Affymetrix, a genetic technology company based in Santa Clara, CA. The gene chip assesses 500,000 genetic markers in a single experiment.
Spindler's team used a gene chip to examine 11,000 genes from the mouse liver.
Washes and staining of the arrays were performed using a fluidics station (Affymetrix Fluidics Station 450; Affymetrix), and images were obtained using a gene-chip scanner (Affymetrix GeneChip Scanner 3000; Affymetrix).
Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
Using a GLYCOv4 gene chip containing approximately 1260 human genes, we observed differential expression of 131 genes.
Key to the system is the use of a gene chip on which thousands of small fragments of the desired DNA sequence are synthesized in parallel in one step.
