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Hygromycin-resistant cell clones were selected using a functional tumor necrosis factor-α (TNF-α) assay that induces luciferase activity.
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These studies require proof of capacity using a functional assay, usually tumor growth in xenograft transplants.
The evaluation of which Gene Ontology (GO) classes are differentially methylated between tumor and surrounding samples was performed using a functional class scoring analysis as previously described [29].
Moreover, a pancreatic endocrine tumor will cause endocrine symptoms if it is a functional tumor.
Therefore, we used a functional approach to investigate whether rhabdomyosarcoma tumors might have a subpopulation enriched in CSCs and are hierarchically organized.
This was accomplished by using a preclinical animal tumor model.
It is not enough to have a presumptive antigen but more relevant to have and use functional, tumor-specific antigens.
Prior analysis of these tumors using functional assays of tumor initiating potential have showed that the tumor-generating cells had a predominantly basal character, and they were enriched in a Thy1+/CD24+ cell sub-population (1% of total Lin− cells, showing 35× enrichment of functional activity) [12].
Participants also completed the Functional Assessment of Cancer Therapy (FACT) quality of life questionnaire using a version specific to their tumor type.
Thus, our results demonstrate that OPCML is a broad functional tumor suppressor that is epigenetically silenced in multiple tumors.
Nor we did use a tumor-resection-type or a custom-made prosthesis to get a more functional and durable outcome.
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