GAS5 (growth arrest-specific transcript 5) was identified using a functional screen through its ability to suppress apoptosis in a mouse thymoma cell line [ 1].
Using a functional screen, we confirmed that hopO1, hopT1, hopAH1, hopM1', hopAE1, hopAR1, and hopAI1' are part of the virulence-associated HrpL regulon, though the hopAI1' and hopM1' sequences were degenerate with premature stop codons.
Here we have used a functional screening assay in mammalian cells to isolate an Arabidopsis complementary DNA clone encoding a Ca(2+ -sensing receptor, CAS.
In this study we generated a draft complete genome sequence of Pta 11528 and used a functional screen for HrpL-dependent genes to infer its repertoire of T3SS effectors and associated Hrp Outer Proteins (Hops), which differs significantly from that of its closest relative whose complete genome has previously been published (Pph 1448A).
Thallium flux was measured at room temperature using a Functional Drug Screening System 7000 FDSS 70000, Hamamatsu).
Calcium flux was measured over time as an increase in fluorescence using a functional drug screening system 6000 FDSS 60000, Hamamatsu, Japan).
Using a functional genomic screen to find host proteins involved in HIV infection; why it is hard to recruit the immune system to fight cancer; Antarctic glaciers during a very warm period of Earth's history; Science Careers on doing what you love; and more.
The second WRE localized to a region 1.6 kb downstream from the MYC transcription stop site and was identified using a functional genomics screen for ß-catenin binding sites [14], [15].
In summary, using a functional HTS screen and subsequent library synthesis approach, we have discovered a novel series of highly selective mGlu5 PAMs and a neutral ligand that have properties that suggest that they do not act as traditional MPEP site ligands.
A functional screening (using the same molecules used for CYP71AJ13 and described in the Additional file 1) performed on these proteins belonging to the group II-6 was unsuccessful thus reinforcing the hypothesis of a function not related to the synthesis of molecules close to coumarins.
