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The specific objective was to measure the effect of glucocorticoid treatment on gene expression of cutaneous antimicrobial peptides that are known to inhibit the in vitro growth of B. dendrobatidis, using a frog species considered susceptible to chytridiomycosis (Lithobates [ Rana] pipiens) [ 15].
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"We use a frog, because it has long been a symbol of fertility, creation, going back to the ancients.
But instead of using rodents, we can slowly go use smaller species, like the zebrafish embryo, for example, or some people use a frog embryo.
1900: Leonard Hill used a frog model to prove that decompression causes bubbles and that recompression resolves them.
Thompson didn't have much taste for door-to-door canvassing or other grunt work, but he was a compelling speaker and created memorable ads that used a frog to symbolize jumping, unstable inflation.
In 1852, H. F. Stannius used a frog's heart in a procedure called a Stannius ligature to demonstrate the ventricle and atria beat independently of each other and at different rates.
After observing the shaking, or tremulation, he tested his hypothesis on the frogs by simulating territorial squabbles, using a robotic frog and a mechanical shaker.
If you're using a flower frog, grid or allowing the flowers to stand on their own, you should add the water to the vessel at this point.
If you're using a flower frog or a cellophane grid (which are good for holding top heavy flowers or those with woody stems), place it into the container and secure with some tape or a little floral adhesive.
In an attempt to excite the crowd, Pinky Downs, a 1906 Texas A&M graduate and member of the school's Board of Regents, asked "What are we going to do to those Horned Frogs?" Using a term for frog hunting, he answered his own question, "Gig 'em, Aggies!" For emphasis, he made a fist with the thumb extended.
We utilised a tsunami simulation tool (Goto and Shuto1983; Shuto1991) that is capable of generating off-shore tsunami propagation and inundation profiles by evaluating nonlinear shallow water equations considering run-up using a leap-frog staggered finite difference scheme.
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