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Air-dried CT (1 kg) was cut into pieces by using a fodder chopper, and the extract was prepared by incubating with 80% methanol at room temperature for several days.
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The laser beam is chopped using a mechanical chopper and directed to the sample surface.
The light beam is passed through a monochromator (Jobin Yvon), modulated using a mechanical chopper (SR540, Stanford research systems) and focused to an airtight photoacoustic (PA) cell.
The hippocampi were dissected free and 400 µm-thick (transverse section in hippocampus) slices were cut using a tissue chopper.
Endometrium was peeled off with tweezers and chopped into 400 × 400 µm wide pieces using a tissue chopper (McIwain tissue chopper, Agar Scientific, Essex, UK).
Then transverse sections (400 μm) were obtained using a tissue chopper.
The direction of illumination was alternated using a mechanical chopper (ThorLabs) at rates up to 750 Hz.
Coronal slices (∼300 μm) were then prepared using a tissue chopper and trimmed to ∼10 mm slices containing both nuclei.
Spinal cord samples were collected and embedded in low-melting point agarose and sectioned in 750 μm thick sections using a tissue chopper (McIlwain Tissue Chopper, The Mickle Laboratory Engineering Company; Goose Green, United Kingdom).
Quasi-DC photocurrent imaging was initially performed by modulating the laser excitation (∼100 200 Hz) using a mechanical chopper and current was detected using a lock-in amplifier.
In the present study, we isolated the forebrain from early postnatal mice and sectioned them sagittally into 350-μm-thick slices by using a tissue chopper.
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