Sentence examples for using a fluorogenic substrate from inspiring English sources
Here, we describe in detail the steps for the microdissection of neonatal cochlea, dissociation of these tissues, labeling of the LacZ-positive cells using a fluorogenic substrate, and cell sorting.
To examine the content of apical proteins in the channel membranes, the bile canalicular enzyme dipeptidyl peptidase IV (DPPIV) was localized using a fluorogenic substrate, Ala-Pro-cresyl violet.
By using a fluorogenic substrate, Z-Arg-Leu-Arg-Gly-Gly-AMC, the deubiquitinating activities for H6YUH1 (1.72 U/mg) and YUH1H6 (1.61 U/mg) were about 18 times higher than 0.092 U/mg for H6UBP1, ubiquitin specific protease 1 of S. cerevisiae containing the 6 histidine residue at the N-terminus which is normally used in protein engineering.
Incubation of the cytosolic S100 cell extract with dATP and Cyt c restored the apoptotic pathway through induction of the apoptosome formation (Fearnhead, 2001); this restoration was followed using a fluorogenic substrate for caspases (Ac-DEVD-afc).
Using a fluorogenic substrate, we did detect a slight decrease in 20S proteasome activity in vitro, but not in 26S proteasome activity – although 20S proteasome does exist in cells, it is thought that the 26S proteasome is responsible for the degradation of ubiquitylated proteins [37].
The activity was measured using a fluorogenic substrate (Fluorescent FXIII Assay; Zedira GmbH, Darmstadt, Germany).
Rimm's alternative is to use a fluorogenic substrate instead, a strategy called "quantitative immunofluorescence" (QIF).
We used a fluorogenic substrate for the β-galactosidase enzyme to quantify enzymatic activity in a Wallac Victor 3 fluorimeter (Methods).
We also compare the sensitivity of this method to that using a fluorogenic peptide substrate, carbobenzoxy-LRGG-7-amido-4-methylcoumarin (Cbz-LRGG-AMC), that has also been used as a substrate for DUBs (19).
Protease assay was performed using a fluorogenic peptide substrate and measured by fluorescence spectrometry.
Lysine deacetylase activity of Sir2 and other enzymes was evaluated using a fluorogenic peptide substrate (Fluor-de-Lys green HDAC drug discovery kit: BML-AK530-0001, Enzo Life Sciences).
