Sentence examples for using a fluorogenic peptide from inspiring English sources

Protease assay was performed using a fluorogenic peptide substrate and measured by fluorescence spectrometry.

The level of MMP-2 activation was determined using a fluorogenic peptide.

Proteasome function was measured in cell lysates from ECV304 cells incubated with different doses of verapamil, doxorubicin, daunorubicin, idarubicin, epirubicin, topotecan, mitomycin C, and gemcitabine using a fluorogenic peptide assay.

Lysine deacetylase activity of Sir2 and other enzymes was evaluated using a fluorogenic peptide substrate (Fluor-de-Lys green HDAC drug discovery kit: BML-AK530-0001, Enzo Life Sciences).

We also compare the sensitivity of this method to that using a fluorogenic peptide substrate, carbobenzoxy-LRGG-7-amido-4-methylcoumarin (Cbz-LRGG-AMC), that has also been used as a substrate for DUBs (19).

The proteolytic activity of CTSB in cells was measured using a fluorogenic peptide substrate, Z-Leu-Arg-AMC, which has been used to assay human cathepsins B, L, and V. CTSB proteolytic activity was markedly lower in cells treated with LVK-CHO, a potent CTSB inhibitor.

To test the possibility that the phosphorylation of SIRT1 might modulate its NAD+-dependent deacetylase activity, we compared the enzymatic activity of phosphorylated and dephosphorylated affinity-purified FLAG-SIRT1 using a fluorogenic peptide-substrate-based assay system, Fluor-de Lys [29].

However, this assay uses a fluorogenic peptide containing 7-amino-4-methylcoumarin (AMC), which becomes the cause of false positive hits from screenings.

This assay measures a distinct protease activity associated with cytotoxicity and uses a fluorogenic peptide substrate (bis-alanyl-alanyl-phenylanlanyl-rhodamine 110 (bis-AAF-R110)) to assess protease activity that has been released from cells that have lost membrane integrity (Promega, Mannheim, Germany).

The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (glycyl-phenylalanyl-aminofluorocoumarin; GF-AFC).

The assay for viability was performed, using a fluorogenic, cell permeant, peptide substrate (glycil-phenylalanyl-amino-fluorocoumarin, [GFAFC], Promega).