Sentence examples for using a fluorogenic nuclease from inspiring English sources

Primers and probe were tested for selectivity, specificity and sensitivity of detection of the target organism using a fluorogenic nuclease assay and a sequence detector.

All PCR amplifications were carried out in duplicate on an ABI Prism® 7300 Sequence Detection System, using a fluorogenic 5' nuclease assay (TaqMan® probes).

Expression levels of Fas mRNA were analyzed by quantitative real-time PCR using a fluorogenic 5'-nuclease assay (TaqMan®; Applied Biosystems, Weiterstadt, Germany) on an ABI Prism 7900 HT Sequence Detection system (Applied Biosystems).

The S-WGA products were genotyped for 23 SNPs, covering all autosomes and X chromosomes, using a fluorogenic 5'-nuclease (Taqman) assay (Table S1).

The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (glycyl-phenylalanyl-aminofluorocoumarin; GF-AFC).

Results were validated through an in vitro DENV2 NS2B/NS3 protease assay using a fluorogenic Boc-Gly-Arg-Arg-AMC substrate.

Rs688 genotyping was performed using a fluorogenic allele-specific amplification method (Millipore) as previously described [6].

Using a fluorogenic substrate, we demonstrated that GST Sir2 is a catalytically active lysine deacetylase.

One scaffold was synthesized and inhibitory binding tested using a fluorogenic ACE assay.

The activity was measured using a fluorogenic substrate (Fluorescent FXIII Assay; Zedira GmbH, Darmstadt, Germany).

Whole blood 20S proteasome activity was measured using a fluorogenic assay, as previously reported.