Sentence examples for using a fluorogenic exonuclease from inspiring English sources

Real-time PCR amplification using a fluorogenic 5' exonuclease assay was performed in triplicate on negative controls and DNA plasmid dilutions of 10 102 copies to give a total of 24 reactions per PCR experiment.

Additionally, we measured ROS levels using a fluorogenic dye-based method that allowed us to approximate the treatment-specific levels of oxidative stress experienced by nematodes [ 47].

The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (glycyl-phenylalanyl-aminofluorocoumarin; GF-AFC).

Results were validated through an in vitro DENV2 NS2B/NS3 protease assay using a fluorogenic Boc-Gly-Arg-Arg-AMC substrate.

Rs688 genotyping was performed using a fluorogenic allele-specific amplification method (Millipore) as previously described [6].

The S-WGA products were genotyped for 23 SNPs, covering all autosomes and X chromosomes, using a fluorogenic 5'-nuclease (Taqman) assay (Table S1).

Using a fluorogenic substrate, we demonstrated that GST Sir2 is a catalytically active lysine deacetylase.

One scaffold was synthesized and inhibitory binding tested using a fluorogenic ACE assay.

The activity was measured using a fluorogenic substrate (Fluorescent FXIII Assay; Zedira GmbH, Darmstadt, Germany).

Whole blood 20S proteasome activity was measured using a fluorogenic assay, as previously reported.

Protease assay was performed using a fluorogenic peptide substrate and measured by fluorescence spectrometry.