Sentence examples for using a fluorogenic competitor from inspiring English sources
The approaches include binding of labeled compounds to microsomal fractions, 1394) reaction of chemicals with glutathione that is monitored using a fluorogenic competitor, and 2D NMR analysis (ALARM NMR) of binding to isotopically labeled human La antigen, whose two cysteins can react with chemicals or change the oxidation state in the presence of chemicals.
The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (glycyl-phenylalanyl-aminofluorocoumarin; GF-AFC).
Results were validated through an in vitro DENV2 NS2B/NS3 protease assay using a fluorogenic Boc-Gly-Arg-Arg-AMC substrate.
Incubation of the cytosolic S100 cell extract with dATP and Cyt c restored the apoptotic pathway through induction of the apoptosome formation (Fearnhead, 2001); this restoration was followed using a fluorogenic substrate for caspases (Ac-DEVD-afc).
Cysteine-aspartic protease-3 (caspase-3) activation was verified by using a fluorogenic NucView 488 Caspase-3/7 substrate for live cells, following the vendor's protocol (Biotium, Hayward, CA).
This sialidase activity is revealed in the periphery surrounding the cells using a fluorogenic sialidase specific substrate, 4-MUNANA [2′- 4-methylyumbelliferyl -α-D-N-acetylneuraminic acid], which fluoresces at 450 nm caused by the emission of 4-methylumbelliferone.
Rs688 genotyping was performed using a fluorogenic allele-specific amplification method (Millipore) as previously described [6].
The S-WGA products were genotyped for 23 SNPs, covering all autosomes and X chromosomes, using a fluorogenic 5'-nuclease (Taqman) assay (Table S1).
Using a fluorogenic substrate, we demonstrated that GST Sir2 is a catalytically active lysine deacetylase.
One scaffold was synthesized and inhibitory binding tested using a fluorogenic ACE assay.
The activity was measured using a fluorogenic substrate (Fluorescent FXIII Assay; Zedira GmbH, Darmstadt, Germany).
