Sentence examples for using a fluorogenic cathepsin from inspiring English sources

Both inhibitors blocked intracellular cathepsin activity as measured using a fluorogenic cathepsin B/L assay (Fig. 4E).

Additionally, we measured ROS levels using a fluorogenic dye-based method that allowed us to approximate the treatment-specific levels of oxidative stress experienced by nematodes [ 47].

The proteolytic activity of CTSB in cells was measured using a fluorogenic peptide substrate, Z-Leu-Arg-AMC, which has been used to assay human cathepsins B, L, and V. CTSB proteolytic activity was markedly lower in cells treated with LVK-CHO, a potent CTSB inhibitor.

Cathepsins (0.03 nM to 60 nM) were assayed in pH 5.5 MES buffer using a fluorogenic substrate Z-Phe-Arg-amido-4-methylcoumarin (10 to 50 μM; Z-Phe-Arg-AMC; Calbiochem) [ 8, 9, 23] in a 96-well plate.

The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (glycyl-phenylalanyl-aminofluorocoumarin; GF-AFC).

Results were validated through an in vitro DENV2 NS2B/NS3 protease assay using a fluorogenic Boc-Gly-Arg-Arg-AMC substrate.

Rs688 genotyping was performed using a fluorogenic allele-specific amplification method (Millipore) as previously described [6].

The S-WGA products were genotyped for 23 SNPs, covering all autosomes and X chromosomes, using a fluorogenic 5'-nuclease (Taqman) assay (Table S1).

Using a fluorogenic substrate, we demonstrated that GST Sir2 is a catalytically active lysine deacetylase.

One scaffold was synthesized and inhibitory binding tested using a fluorogenic ACE assay.

The activity was measured using a fluorogenic substrate (Fluorescent FXIII Assay; Zedira GmbH, Darmstadt, Germany).