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Cells were washed once in phenol red free media and images captured using a Zeiss Axioplan fluorescent microscope or fluorescence quantified using a fluorescent plate reader.
Fluorescence was determined using a fluorescent plate reader (*P < 0.05, n = 6).
The beads were washed and the captured fluorescence was measured using a fluorescent plate reader (Gemini XS, Molecular Probes).
After NSPCs were incubated with 10 μM DCFH-DA at 37 °C for 30 min, the fluorescence was monitored by scanning the whole well using a fluorescent plate reader at excitation and emission wavelengths of 485 and 525 nm.
Relative fluorescence intensity was recorded over time 3-1200 min) by using a fluorescent plate reader (Thermo, Appliskan) at an excitation wavelength of 485 nm and emission was measured at a wavelength of 530 nm, and fluorescent images were also obtained by using a fluorescence microscopy (ZEISS, Axiovert 200 M).
The fluorescence released following proteolytic cleavage of the substrate was determined using a fluorescent plate reader.
Similar(31)
Proteolytic activities were assessed in 2 h at 25°C by measuring the release of the fluorescent group AMC using a fluorescence plate reader (Fluoroskan Ascent, Thermo Scientific, Waltham, MA, USA) with excitation and emission wavelengths of 380 and 460 nm, respectively.
The fluorescent intensity was measured using a fluorescence plate reader (BIO-TEK FL600, BIO-TEK instruments, Inc., Winooski, USA) after 20 h of incubation at 37°C.
After 24 hours, all aliquots taken and contained in the 96-well plate were examined using a fluorescent 96-well plate spectrometer (Spectra Max 190 Absorbance UV VIS plate reader) at an excitation wavelength of 485 nm and the fluorescent intensity measured at the emission wavelength of 535 nm.
The labeling solution was removed and the plates washed twice, and we then counted the tubes in the plate using a fluorescent microscope.
Absorbance of AlamarBlue was measured in a Wallac Victor plate reader using a fluorescent excitation wavelength of 544 nm and fluorescence emission at 590 nm.
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