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The DNA concentration in intact control supernatants was undetectable using a fluorescence spectroscopy assay with ethidium bromide as a fluorescent dye.
The quantification of Cr3+ by 1 using a fluorescence spectroscopy method was satisfactory in the linear working range below 4 μM, with a detection limit of 3.75×10 9 M for Cr3+ and good tolerance of other metal ions.
Using a fluorescence spectroscopy methodology, we did not find a significant difference between the fibrinogen specific affinities to the different erythrocytes population.
Using a fluorescence spectroscopy method they demonstrate that residues outside of the established DBD are not only involved in binding but increase affinity by 5-fold using y-shaped DNA junctions [ 44].
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The number of PAP channels per polymersome was counted using a fluorescence correlation spectroscopy (FCS -based technique reported previously13 and illustrated in Supplementary FCS -based Supplementechniqueods.
Using a fluorescence polarization spectroscopy assay, previously used for studying binding of small molecules to the CUG-RNA hairpin structure (García-López et al., 2011), we identified some hits that exhibited this interesting aptitude (Fig. 6D).
Using a fluorescence fluctuation method (fluorescence correlation spectroscopy, FCS), we found that truncation of the EphA2 receptor by the SAM domain leads to increased oligomerization compared to the full length receptor, and causes constitutive activation of EphA2.
The OCT1-mediated uptake of tropisetron, debrisoquine, O-desmethyltramadol, and monocrotaline was measured using HPLC, of ASP+ using fluorescence spectroscopy and of radiolabelled MPP+, TEA+, morphine, metformin, and tyramine using scintillation counting.
Leaching studies are conducted using naphthalene-conjugated oligostyrenes using fluorescence spectroscopy.
ROS production was confirmed by using DCFH-DA method using fluorescence spectroscopy.
Furthermore, it has been shown that the fatigue threshold R-curve can equivalently be determined by measuring the bridging stress distribution, in this case using fluorescence spectroscopy, using only a long crack compact tension specimen without the need for difficult small crack experiments.
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